are red traces from two images comparable?

[XiaoqianSun0104]

Hi,

I have two recordings from two channels (Ch1 and Ch2) of same ROI: - Neurons in Ch1 and Ch2 have certain level of overlap. - Neurons in Ch1 have normal activity, while neurons in Ch2 should have a stable but weak signal

After using CaImAn to process Ch1/Ch2 recordings, we believe that Ch1 signals leaked to Ch2 since neurons at same location have similar shape of signals but different intensities (neuron in Ch2 should have a static signal).

I noticed that - case1: baseline level sof some Ch2 neurons are higher than same neurons in Ch1. E.g., neuron 87 in Ch1 VS neuron 52 in Ch2 as shown in the attached pic. - case 2: baseline levels of same neurons in Ch1/Ch2 are about the same. E.g., neuron 246 in Ch1 VS neuron 64 in Ch2.

I wonder if these two signals are comparable? Can I come up with a conclusion: signals of Ch2 got pushed up because of the original signals of neuron in Ch2? Thank you for you help!

case 1:

case2: