pavian
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Support for mOTUs2
Hi @fbreitwieser,
I got a request from one of your users to use Pavian with profiles from mOTUs2. See mOTUs_issue_27.
Pavian seems a great tool and I would also like to visualize mOTUs2 profiles on it. Moreover, it would be easy to adapt it from the MetaPhlAn input parser.
When running mOTUs with -A
, you will get as result:
#mOTUs2_clade test1
k__Bacteria 1.0000000000
k__Bacteria|p__Proteobacteria 0.0732714431
k__Bacteria|p__Firmicutes 0.6479060231
k__Bacteria|p__Thermodesulfobacteria 0.0055578606
k__Bacteria|p__Actinobacteria 0.2200557062
k__Bacteria|p__Chlamydiae 0.0532089670
k__Bacteria|p__Proteobacteria|c__Gammaproteobacteria 0.0557659685
k__Bacteria|p__Proteobacteria|c__Alphaproteobacteria 0.0175054746
k__Bacteria|p__Firmicutes|c__Erysipelotrichia 0.0688211617
k__Bacteria|p__Firmicutes|c__Negativicutes 0.0234955832
k__Bacteria|p__Firmicutes|c__Bacilli 0.4538976397
k__Bacteria|p__Firmicutes|c__unknown Firmicutes 0.1016916385
...
k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Corynebacteriales|f__Corynebacteriaceae|g__Corynebacterium|s__Corynebacterium sp. GD7 [ref_mOTU_v2_2683] 0.0390911725
k__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Vibrionales|f__Vibrionaceae|g__Vibrio|s__Vibrio tubiashii [ref_mOTU_v2_3913] 0.0044390411
k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Streptomycetales|f__Streptomycetaceae|g__Streptomyces|s__Streptomyces durhamensis [ref_mOTU_v2_4783] 0.0725347907
The only differences with MetaPhlAn2 are:
- the header, your parser requires
#SampleID\tMetaphlan2_Analysis
. mOTUs profiles have a header like#mOTUs2_clade\tsample_name
wheresample_name
change every time; - MetaPhlAn abundances sum up to 100, while mOTUs abundances sum up to 1.
Cheers, alessio