Question: may I use single-cell sequencing data to make a "pesudo normal sample reference"?

[SenLiBio]

Hi,

Thank you so much for your effort in developing and maintaining such a wonderful tool!

I am currently working on using WGS sequencing data for CNV calling, but I don’t have any normal samples to build a reference. However, I have some single-cell sequencing data from the same biopsy, including normal cells. I’m wondering if it would be beneficial to create a pseudo-normal sample reference by combining some of the single-cell sequencing samples. What do you think?

Thank you for your time!

Best, Sen