Bias corrections

[MicrobioSee]

I got the copy number of the target gene by the DPCR experience, and the result is 8. There are 4 normal and 2tumor samples.

I try to use the "batch" script in cnvkit, and I got the copy number of the target gene, it is 3. I attempt to use cnvkit by simple script, and after using the "--no-edge" and "--no-gc", the result is 10.

I want to know that If I got the result with "--no-edge" and "--no-gc", is the result obtained in this way meaningful？