`calculate` error when using `-p blastp`

[bluegenes]

Thanks for your help with the previous issue - I'm back with a different one!

I'd like to use blastp for some AAI calculations. I used conda to install blastp in the same environment I was previously using for EzAAI calculations (info below). I'm running a single instance at a time.

As I understand it, I use the same extract command to prepare databases, regardless of mapping type - is that incorrect?

Using the sample data from your tutorial:

java -jar EzAAI_v1.11.jar calculate -i db/Ci.db -j db/Lc.db \
      -t 10 -p blastp -o test.blastp.tsv -v

I get the following error:

[FEB 24 08:51:15] EzAAI   |:  EzAAI - v1.11 [Feb. 2022]
getEngineName() called
[FEB 24 08:51:16] EzAAI   |:  Calculating AAI... [Task 1/1]
[FEB 24 08:51:16] EzAAI   |:  Preparing to run reciprocal BLASTp+...
[FEB 24 08:51:16] EzAAI   |:  Running BLASTp+... (/tmp/bdd397448b571442_faa/i0.faa vs. /tmp/bdd397448b571442_faa/j0.faa)
java.io.FileNotFoundException: /tmp/bdd397448b571442_vice.out (No such file or directory)
        at java.base/java.io.FileInputStream.open0(Native Method)
        at java.base/java.io.FileInputStream.open(FileInputStream.java:219)
        at java.base/java.io.FileInputStream.<init>(FileInputStream.java:157)
        at java.base/java.io.FileReader.<init>(FileReader.java:75)
        at leb.process.ProcFuncAnnoByBlastPlus.parseOutFile(ProcFuncAnnoByBlastPlus.java:283)
        at leb.process.ProcFuncAnnoByBlastPlus.execute(ProcFuncAnnoByBlastPlus.java:273)
        at leb.process.ProcCalcPairwiseAAI.pairwiseBlastp(ProcCalcPairwiseAAI.java:499)
        at leb.process.ProcCalcPairwiseAAI.calculateProteomePairWithDetails(ProcCalcPairwiseAAI.java:248)
        at leb.main.EzAAI.runCalculate(EzAAI.java:361)
        at leb.main.EzAAI.run(EzAAI.java:493)
        at leb.main.EzAAI.main(EzAAI.java:528)
[FEB 24 08:51:16] ERROR   |:  Program terminated with error.

Please let me know if you have ideas on how to proceed. Thanks!

Conda environment:

conda list -p /home/ntpierce/miniconda3/066c58eb7ac25d2ab6a302135cb3ad40
# packages in environment at /home/ntpierce/miniconda3/066c58eb7ac25d2ab6a302135cb3ad40:
#
# Name                    Version                   Build  Channel
_libgcc_mutex             0.1                 conda_forge    conda-forge
_openmp_mutex             4.5                       1_gnu    conda-forge
blast                     2.12.0               h3289130_3    bioconda
bzip2                     1.0.8                h7f98852_4    conda-forge
c-ares                    1.18.1               h7f98852_0    conda-forge
ca-certificates           2021.10.8            ha878542_0    conda-forge
curl                      7.81.0               h494985f_0    conda-forge
entrez-direct             16.2                 he881be0_0    bioconda
gawk                      5.1.0                h7f98852_0    conda-forge
gettext                   0.19.8.1          h73d1719_1008    conda-forge
krb5                      1.19.2               h48eae69_3    conda-forge
libcurl                   7.81.0               h494985f_0    conda-forge
libedit                   3.1.20191231         he28a2e2_2    conda-forge
libev                     4.33                 h516909a_1    conda-forge
libffi                    3.4.2                h7f98852_5    conda-forge
libgcc-ng                 11.2.0              h1d223b6_12    conda-forge
libgomp                   11.2.0              h1d223b6_12    conda-forge
libidn2                   2.3.2                h7f98852_0    conda-forge
libnghttp2                1.46.0               ha19adfc_0    conda-forge
libssh2                   1.10.0               ha35d2d1_2    conda-forge
libstdcxx-ng              11.2.0              he4da1e4_12    conda-forge
libunistring              0.9.10               h7f98852_0    conda-forge
libzlib                   1.2.11            h36c2ea0_1013    conda-forge
mmseqs2                   13.45111             h95f258a_1    bioconda
ncurses                   6.3                  h9c3ff4c_0    conda-forge
openssl                   3.0.0                h7f98852_2    conda-forge
pcre                      8.45                 h9c3ff4c_0    conda-forge
perl                      5.32.1          1_h7f98852_perl5    conda-forge
perl-archive-tar          2.40            pl5321hdfd78af_0    bioconda
perl-carp                 1.50            pl5321hd8ed1ab_0    conda-forge
perl-common-sense         3.75            pl5321hdfd78af_0    bioconda
perl-compress-raw-bzip2   2.101           pl5321h1b792b2_0    bioconda
perl-compress-raw-zlib    2.101           pl5321h1b792b2_1    bioconda
perl-encode               3.16            pl5321h779adbc_0    bioconda
perl-exporter             5.74            pl5321hd8ed1ab_0    conda-forge
perl-exporter-tiny        1.002002        pl5321hdfd78af_0    bioconda
perl-extutils-makemaker   7.64            pl5321hd8ed1ab_0    conda-forge
perl-io-compress          2.102           pl5321h1b792b2_0    bioconda
perl-io-zlib              1.11            pl5321hdfd78af_0    bioconda
perl-json                 4.05            pl5321hdfd78af_0    bioconda
perl-json-xs              2.34            pl5321h7d875b9_4    bioconda
perl-list-moreutils       0.430           pl5321hdfd78af_0    bioconda
perl-list-moreutils-xs    0.430           pl5321h779adbc_0    bioconda
perl-parent               0.238           pl5321hd8ed1ab_0    conda-forge
perl-pathtools            3.75            pl5321h779adbc_2    bioconda
perl-scalar-list-utils    1.60            pl5321h779adbc_0    bioconda
perl-types-serialiser     1.01            pl5321hdfd78af_0    bioconda
prodigal                  2.6.3                h779adbc_3    bioconda
tk                        8.6.11               h27826a3_1    conda-forge
wget                      1.20.3               ha35d2d1_1    conda-forge
zlib                      1.2.11            h36c2ea0_1013    conda-forge