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Bug in STARFISH annotate code

Open drdna opened this issue 6 months ago • 1 comments

There is a bug in the code that is preventing execution of the bedtools intersect command:

[Mon Aug 5 21:10:14 2024] checking formatting of GFFs in ome2gff.txt.. sh: -c: line 0: syntax error near unexpected token (' sh: -c: line 0: bedtools intersect -a CD156_SF_starships/CD156.filt.gff -b <(grep -w mRNA /scratch/farman/STARFISH/CD156_processed.gff) -wao >> CD156_SF_starships/CD156.intersect.gff'

[Mon Aug 5 21:10:14 2024] error: could not execute bedtools intersect on commandline for CD156_SF_starships/CD156.filt.gff and /scratch/farman/STARFISH/CD156_processed.gff, exiting.. No such file or directory

Troubleshooting is complicated by the fact that the error message is incorrect. Both files referenced in the message are present and correct in the specified locations. However, bedtools intersect is looking for a temp.gff that, apparently, is never created.

Note: This step also fails with the starfish examples datasets.

drdna avatar Aug 06 '24 01:08 drdna