Ed Lui

> This can be done in Unix like this: > > ``` > cat in.fq | paste - - - - | grep -v -f -F name.lst | tr "\t"...

in my case, it is due to min_split_read_size after splitting the middle adapters. by default --min_split_read_size is 1000, after splitting if read length < 1000, then the reads are removed.