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Error in processTrajectories

Open cjpark85 opened this issue 5 years ago • 8 comments

Hi, I'm following the tutorials and issues in github to make trajectory using Seurat object. I got this error from processTrajectories.

filename <- "results/paths/cell_edge_weighted_network.txt" write.table(cellrouter@graph$edges, file=filename, sep='\t', row.names=FALSE, col.names = FALSE, quote=FALSE) #Select cluster 11 sources <- c('11') targets <- setdiff(as.vector(cellrouter@sampTab$seurat_clusters), sources) methods <- c("euclidean", "maximum", "manhattan","canberra","binary", 'graph') #graph for distances in KNN ##Identify trajectories cellrouter <- findPaths(cellrouter, column='seurat_clusters', libdir, paste(getwd(), 'results/paths', sep='/'), method="graph") -------------------Transition: 11.0 ----------------------- -------------------Transition: 11.2 ----------------------- -------------------Transition: 11.3 ----------------------- -------------------Transition: 11.4 ----------------------- -------------------Transition: 11.5 ----------------------- -------------------Transition: 11.6 ----------------------- cellrouter <- processTrajectories(cellrouter, rownames(cellrouter@ndata), path.rank=ranks[3], num.cells = 3, neighs = 3, column.ann = 'seurat_clusters', column.color = 'seurat_clusters_colors') [1] "parsing trajectory information" Error in file(file, "rt") : cannot open the connection In addition: Warning message: In file(file, "rt") :

cjpark85 avatar May 08 '19 18:05 cjpark85