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Methylation profile ERROR for large genome

Open Yang990-sys opened this issue 4 months ago • 1 comments

Hello, Help please,

ERROR: can't load csi index,try to rebuild bai index and report bug

Image

MethylDackel version: 0.6.1 (using HTSlib version 1.21) Installed by conda

script:

bsmap -a sample.R1.clean.fq.gz  -b  sample.R2.clean.fq.gz -d ref_bis/all.fa -p 20 -v 0.05 -o align.bam 2>bsmap_report.txt

sambamba sort -m 8GB --tmpdir tmp -t 10 -o ${prefix}_sorted.bam ${prefix}.bam
sambamba markdup --overflow-list-size 1000000 --tmpdir tmp -t 10 ${prefix}_sorted.bam dedup.bam 2> markdup_report.txt

MethylDackel extract ref_bis/all.fa dedup.bam --opref prefix

Thanks a lot

Yang990-sys avatar Aug 14 '25 04:08 Yang990-sys