Could not repeat a CpG extraction with the same reference file and its index

[sttongjai]

Hello,

I was using MethylDackel via Conda to extract CpG from a BAM file created by bwa-meth. After my first attempt of extraction CpG which was successful, my second attempt, aiming at repeating the previous extraction, was not.

I received an error message telling that the program could neither load an index file of a reference genome (hg38 with no alternative chromosomes) nor build one for it. It also suggested that the reference genome file could be corrupted. However, the index file from the previous run was in the same directory as the reference genome.

Could you please let me know what's a solution to this problem.

Thank you very much,

Siripong

P.S. Please see a list of programs and their versions installed in my conda environment below.

Name Version Build Channel

_libgcc_mutex 0.1 main
_openmp_mutex 5.1 1_gnu
bzip2 1.0.8 h7b6447c_0
c-ares 1.19.0 h5eee18b_0
ca-certificates 2023.05.30 h06a4308_0
htslib 1.12 h9093b5e_1 bioconda krb5 1.19.4 h568e23c_0
libcurl 7.88.1 h91b91d3_0
libdeflate 1.17 h5eee18b_0
libedit 3.1.20221030 h5eee18b_0
libev 4.33 h7f8727e_1
libgcc-ng 11.2.0 h1234567_1
libgomp 11.2.0 h1234567_1
libnghttp2 1.52.0 ha637b67_1
libssh2 1.10.0 h37d81fd_2
libstdcxx-ng 11.2.0 h1234567_1
methyldackel 0.6.1 h22771d5_0 bioconda ncurses 6.4 h6a678d5_0
openssl 1.1.1u h7f8727e_0
xz 5.2.10 h5eee18b_1
zlib 1.2.13 h5eee18b_0