Issue with sigclus

[danieljrichard]

Hello, I've been trying to run 'sigclus' with the demo data provided as well as the pre-processed proximity files. However - I've run into a number of issues. Firstly, if you try running sigclus with the pre-processed proximity files, you get an error like: Can't use an undefined value as an ARRAY reference at /home/daniel/perl5/lib/perl5/TGI/Mutpro/Main/Significance.pm line 461 Which when digging into looks like Significance.pm assumes a single entry for the final column in the proximity file, but there can sometimes be multiple entries. If you force there to be one entry in the final column, and re-run sigclus one gets weird behaviour: Half the time you'll get an error like 'Illegal division by zero at /home/daniel/perl5/lib/perl5/TGI/Mutpro/Main/Significance.pm line 504.' And the other half of the time the program will spit out p-values for some of the clusters, but not others. Most concerningly, if you try running 'sigclus' enough times, it will eventually spit out results - the error behaviour is not consistent.

Is this just a consequence of this function being in 'beta' mode currently? Or am I doing something wrong?

[Beifang]

http://niulab.scgrid.cn/HotSpot3D/ you can try this webserver.