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Does alignmentSieve not support single-ended sequencing data?
Welcome to deepTools GitHub repository! Before opening the issue please check that the following requirements are met :
- [x] Search whether this issue (or a similar issue) has been solved before using the search tab above. Link the previous issue if appropriate below. https://www.biostars.org/p/9582427/#9598279
- [x] Paste your deepTools version (
deeptools --version
) and your python version (python --version
) below. deeptools 3.5.5 Python 3.10.12 - [x] Paste the full deepTools command that produces the issue below
(ignore if you simply spotted the issue in the code/documentation).
alignmentSieve -p ${THREADS} --shift 4 -5 --blackListFileName ${BLACKLIST} -b ${BOWTIE2}.clean.bam -o ${BOWTIE2}.shift.tmp
- [x] Paste the output printed on screen from the command that produces the issue below (ignore if you simply spotted the issue in the code/documentation). no error. but the ${BOWTIE2}.shift.tmp haven't reads infomation
my input data is single-end data. when i use
alignmentSieve -p ${THREADS} --ATACshift --blackListFileName ${BLACKLIST} -b ${BOWTIE2}.clean.bam -o ${BOWTIE2}.shift.tmp
i can get right result. Is it possible for alignmentSieve to support single-ended data?
If not, what should I do?