ISSUES for scaling calculation

[jiangshan529]

Hi, Dear team members,

I am trying to use scaling method in computaMatrix to plot PRO-seq signal from TSS to TES. However, the signal in the gene body(which is the scaled region) is so low, I am wondering if there's any parameters make the signal the same as shown in the original bigwig file. For example, the PRO-seq reads on the gene body is still very strong, but in the scaling plot, the gene body reads is so low.