Why enrichment of TSS and TES region are similar when using scale-region

[haojin9288]

Hello,

While using plotProfile to create enrichment for a Pol cut-tag dataset, I got similar enrichment peak around TSS and TES which actully will show higher enrichment in TSS than TES. So could someone help me figure out what wrong in that: me:

Here is my codes: computeMatrix scale-regions -p 80 --skipZeros -R xx.bed -S xx.bw -b 2500 -a 2500 --regionBodyLength 2500 -o xx.gz plotProfile -m xx.gz -out 1xx.pdf --legendLocation upper-right --dpi 750 --perGroup --averageType mean

Thank you in advance of the help!

[qqss8860]

Probably your bed file is not in the correct format. The strand info has to be in the 6th column, otherwise deepTools won't recognize.