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--filterRNAstrand usage for alignmentSieve is missleading

Open VeredKunik opened this issue 2 years ago • 0 comments

--filterRNAstrand documentation is misleading.

The actual filtration, from what I've tested, actually works as documented for bamCoverage --filterRNAstrand (see bamCoverage --filterRNAstrand.

It indicates this: Selects RNA-seq reads (single-end or paired-end) in the given strand. (Default: %(default)s),

but works like this: Selects RNA-seq reads (single-end or paired-end) originating from genes on the given strand. This option assumes a standard dUTP-based library preparation (that is, --filterRNAstrand=forward keeps minus-strand reads, which originally came from genes on the forward strand using a dUTP-based method)

VeredKunik avatar Aug 30 '23 08:08 VeredKunik