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Understanding bamCoverage output
Hello,
I used bamCoverage with RPKM normalization to get bigwig files. I then used pyBigWig.values()
function to view bigwig files in a given genomic region. I noticed all the non-zero entries in the bigwig are integers. I'm worried that these integers are not normalized values but just raw read counts pile up?
My question is can I safely use pyBigWig.values()
to extract normalized bigwig values for each bp in the region that I'm interested in?
Thanks so much!