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Overlapping and cross nuclei segmentation

Open chenyhvvvv opened this issue 4 months ago • 1 comments

Hi! Thank you for your excellent tools.

/home/yhchenmath/.cargo/bin/proseg --output-spatialdata /import/home2/yhchenmath/Code/Triplet/Data/XeniumHumanLungPreview/output.zarr --xenium /import/home2/yhchenmath/Dataset/DeconvSeg/xenium_human_lung_preview/transcripts.csv

And I get a bad and cross nuclei segmentation with spatialdata visualization. Is there something wrong with my trial?

Image

chenyhvvvv avatar Aug 15 '25 06:08 chenyhvvvv

Hi, thanks for using proseg. Version 3 was was inadvertently outputing a different polygon estimation (unioned polygons) than version 2 (consensus 2d polygons), which made the the overlap seem worse than it is in visualizations. I've just release 3.0.3 which should fix that.

I should also note that your visualization here isn't quite right. Proseg segments using voxels so its boundaries are always made up of horizontal and vertical line segments.

dcjones avatar Aug 15 '25 23:08 dcjones