ERROR: external program called by OrthoFinder returned an error code: 132

[ruthie-7]

It´s my first time working with OrthoFInder and I run the program and this is the answer, I don´t know what to do. I´ve already install all dependencies.

Using 2 thread(s) 2021-12-03 00:09:24 : This may take some time.... 2021-12-03 00:09:24 : Done 0 of 36

ERROR: external program called by OrthoFinder returned an error code: 132

Command: diamond blastp -d /home/ubuntu/OrthoFinder/orthofinder_tutorial/proteomes/primary_transcripts/OrthoFinder/Results_Dec03/WorkingDirectory/diamondDBSpecies0 -q /home/ubuntu/OrthoFinder/orthofinder_tutorial/proteomes/primary_transcripts/OrthoFinder/Results_Dec03/WorkingDirectory/Species2.fa -o /home/ubuntu/OrthoFinder/orthofinder_tutorial/proteomes/primary_transcripts/OrthoFinder/Results_Dec03/WorkingDirectory/Blast2_0.txt --more-sensitive -p 1 --quiet -e 0.001 --compress 1

stdout

stderr

Illegal instruction (core dumped)

[davidemms]

Hi

Sorry for the delayed response. This looks like an error in DIAMOND on your machine. Can you try running the command it show (without the --quiet option) and see what it says: diamond blastp -d /home/ubuntu/OrthoFinder/orthofinder_tutorial/proteomes/primary_transcripts/OrthoFinder/Results_Dec03/WorkingDirectory/diamondDBSpecies0 -q /home/ubuntu/OrthoFinder/orthofinder_tutorial/proteomes/primary_transcripts/OrthoFinder/Results_Dec03/WorkingDirectory/Species2.fa -o /home/ubuntu/OrthoFinder/orthofinder_tutorial/proteomes/primary_transcripts/OrthoFinder/Results_Dec03/WorkingDirectory/Blast2_0.txt --more-sensitive -p 1 -e 0.001 --compress 1

If you've downloaded OrthoFinder directly from github then diamond will be in its 'bin' directly so you'll need to change "diamond" the start of the command to something like "./OrthoFinder/bin/diamond", or whatever the path is on your computer. If you've used conda then the above command should work as is.

Best wishes David