loading tiff files

[annie2013]

I am currently running suite2p on matlab. There seems to be a limit of processing data up to 64000 frames. Is there a way to increase that?

[marius10p]

There is no such limit, but you might have problems with <16GB RAM when you have a lot of neural frames. Another possibility is that all your frames are in a single file (and we don't have incremental tiff loading in the Matlab version yet, but we have it in the Python version).

[annie2013]

I do have sufficient RAM, although all my frames are in a single file. I suppose that the best way to fix this for now in matlab is to seperate the single file to a couple of smaller ones and save them in the same folder?

[horsto]

Hi @annie2013, I had the same problem recently (tifs were apparently getting too big, suite2p didn't spit out a warning, realized much later that files got truncated during processing). Splitting the big tif into multiple small parts helped as you suggested.