comprna
What to do with ioe files with multiple samples?
Hi @EduEyras @leipzig
The data I used is ONT DRS data, there are 2 species (cultivar and wild species, uniformly aligned to the cultivar genome) with 2 replicates and a total of 3 periods, here I want to compare the variable splicing between these two species and different periods of the same species, but I was able to generate the GTF with Isoquant for each sample, but if I were to use isoquant to input all the samples it would also yield A total GTF, but the total GTF is missing some information.
totol ioe
sampl1.rep1
sample1.rep2
sample2.rep1
When running this suppa.py diffSplice you need to type -i ioe
If it is the input total GTF file produces ioe missing some information, but I use the respective samples and when I compare the wild species and cultivars at 5 days there are 4 ioe, what should I do about this situation, please.
