SUPPA
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diffSplice error,a classic one: 'UnboundLocalError'. I have tried my best.
Dear author,
classical
method works fine by add nan_th
to functions classical_test
and calculate_delta_psi
in diff_tools.py
.
But empirical
method still throw the same error, altho
I have reviewed all the issues regarding diffSplice errors, but there is not a solution.
Calculating differential analysis between conditions: TGFb and NC
ERROR:__main__:Unknown error: (<class 'UnboundLocalError'>, UnboundLocalError("local variable 'i' referenced before assignment",), <traceback object at 0x7f5063d2e748>)
My env
* CentOS 7
* python 3.4.5
* conda 4.10.3
* suppa 2.3
My command
suppa.py diffSplice -m empirical -i events.ioi -e NC.tpm.txt TGFb.tpm.txt -p NC.psi.txt TGFb.psi.txt -gc -o diff
My files list below
# NC.tpm.txt
SRR20637415 SRR20637416 SRR20637417
ENST00000000233.10 146.746668972396 149.102385081347 177.659704455211
ENST00000000412.8 104.732062503121 87.2341755040248 91.4535187490958
ENST00000001008.6-0 31.66318168699 18.5604628731968 24.7374272026243
ENST00000002125.9-0 0 0 1.49923801228026
# TGFb.tpm.txt
SRR20637407 SRR20637408 SRR20637409
ENST00000000233.10 146.864611321098 128.127041433003 161.482163166439
ENST00000000412.8 111.571565189672 114.872519905451 111.127510136044
ENST00000001008.6-0 25.6159205792613 30.2960492058333 32.4121904563463
ENST00000002125.9-0 0.569242679539141 1.89350307536458 0
# NC.psi.txt
SRR20637415 SRR20637416 SRR20637417
ENST00000000233.10 0.971774167994478 0.983673443537103 0.991631777070178
ENST00000000412.8 0.868686804929113 0.870370293507435 0.841379242137375
ENST00000001008.6-0 0.981132045654475 0.999999946122039 0.9428571090985
ENST00000002125.9-0 1.09485661410626e-07 2.30905251524717e-07 0.222222172814451
# TGFb.psi.txt
SRR20637407 SRR20637408 SRR20637409
ENST00000000233.10 0.977272701411922 0.975961508908504 0.978947344810134
ENST00000000412.8 0.886877765505706 0.874999940967174 0.914285647291526
ENST00000001008.6-0 0.937499967976461 0.923076897295838 0.933333308377018
ENST00000002125.9-0 0.142857178708518 0.199999978875147 1.32903355558565e-07
# ioi
seqname gene_id isoform_id inclusion_transcripts total_transcripts
chr1 ENSG00000233750.3 ENSG00000233750.3;ENST00000442987.3 ENST00000442987.3 ENST00000442987.3
chr1 ENSG00000225972.1 ENSG00000225972.1;ENST00000416931.1 ENST00000416931.1 ENST00000416931.1,SRR20637415.6316085-0
chr1 ENSG00000225972.1 ENSG00000225972.1;SRR20637415.6316085-0 SRR20637415.6316085-0 ENST00000416931.1,SRR20637415.6316085-0
chr1 ENSG00000225630.1 ENSG00000225630.1;ENST00000457540.1 ENST00000457540.1 ENST00000457540.1
chr1 ENSG00000229344.1 ENSG00000229344.1;ENST00000427426.1 ENST00000427426.1 ENST00000427426.1
chr1 ENSG00000240409.1 ENSG00000240409.1;ENST00000467115.1 ENST00000467115.1 ENST00000467115.1
chr1 ENSG00000248527.1 ENSG00000248527.1;ENST00000514057.1 ENST00000514057.1 ENST00000514057.1
chr1 ENSG00000268663.1 ENSG00000268663.1;ENST00000438434.2 ENST00000438434.2 ENST00000438434.2
chr1 ENSG00000237491.10 ENSG00000237491.10;ENST00000592547.1 ENST00000592547.1 ENST00000592547.1