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"Differential transcript usage", the cognate gene expression?

Open iamhealthy opened this issue 10 months ago • 1 comments

Hi,

My interest is "differenctial transcript usage".

My question is: How could I obtain differential gene (not transcript) expression? Using (the formatted) iso_tpm file? Then I could analyze the differential transcripts and genes together. Could you please give me some tips?

Many thanks!

iamhealthy avatar Apr 05 '24 11:04 iamhealthy

Please have a look at tximport: https://github.com/thelovelab/tximport to summarise the transcript quantification per gene to perform differential gene expression Best Eduardo

On Fri, 5 Apr 2024 at 22:49, iamhealthy @.***> wrote:

Hi,

My interest is "differenctial transcript usage".

My question is: How could I obtain differential gene (not transcript) expression? Using (the formatted) iso_tpm file? Then I could analyze the differential transcripts and genes together. Could you please give me some tips?

Many thanks!

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EduEyras avatar Apr 13 '24 14:04 EduEyras