When calculating the cluster, the following prompt appears, and only a 2kb result file is generated. Is this a data problem? Still reporting an error?

[ouyang-lang]

Running OPTICS clustering...100%
Number of clustered events: 0/19 (0.000000%) Impossible to calculate silhouette score. Only 1 cluster group identified.

When calculating the cluster, the following prompt appears, and only a 2kb result file is generated. Is this a data problem? Still reporting an error?

[ouyang-lang]

suppa.py clusterEvents --dpsi oocyte_diffSplice.dpsi --psivec oocyte_diffSplice.psivec --sig-threshold 0.05 --eps 0.05 --min-pts 20 --groups 1-11,12-21 -o test

This is my command, the result shows that only one group can be recognized

[EduEyras]

Hi,

If there is only one cluster the silhouette score cannot be calculated, as it relies on inter cluster distances

Perhaps you can explore other parameters to see whether more clusters can be obtained

Sometimes it helps starting with some relaxed parameters to allow more events to cluster

I hope this helps

E

On Thu, 14 Oct 2021 at 19:03, ouyang-lang @.***> wrote:

suppa.py clusterEvents --dpsi oocyte_diffSplice.dpsi --psivec oocyte_diffSplice.psivec --sig-threshold 0.05 --eps 0.05 --min-pts 20 --groups 1-11,12-21 -o test

This is my command, the result shows that only one group can be recognized

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