Determination of uniformity and periodicity thresholds

[marcasriv]

Dear authors,

I've a question related to the procedure that you followed to classify translated and untranslated ORFs after finishing running ORQAS. How do you exactly determine the cut-off values for the uniformity and periodicity? Also, I've noticed that in Supplementary Figure 1 each different dataset presents different cutoffs, therefore do cutoffs need to be re-calculated for each dataset?

Thanks so much,

Marina