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RAM required for bacterial genome assembly from HiFi reads
Hi I tied to assemble a bacterial genome (about 3MB) from PacBio Sequel II HiFi reads (about 1 million reads, mean length 6500bp) using a work station with 16 threads and 96GB RAM. Hifiasm maxed out all CPU threads and RAM after second round and system froze. I could not recover the system and had to hard restart (power off, start). I jut want to know if 96GB RAM is enough to assemble bacterial genomes from HiFi reads? Thanks Gurjit