hifiasm
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Accuracy in regions with only ONT support?
One of the key benefits of adding ONT to a PacBio HiFi assembly is to fill in the all the gaps arising from GA(n) coverage drop outs. Thus, in these regions there will only be ONT coverage. How does hifiasm generate the consensus sequence in this region given that it does not have an explicit consensus step? Is a single ONT read being used to span this region? Just want to confirm.
Below are the 25x HiFi reads (top) and 45x ONT (bottom) data aligned to the assembly with a GA(n) repeat region being shown.