gatk icon indicating copy to clipboard operation
gatk copied to clipboard

Problem with PathSeqPipelineSpark : Not generating bam

Open martitroiani opened this issue 1 year ago • 0 comments

Hello, I'm using gatk 4.2.2.0 with PathSeqPipelineSpark but I'm having some problems. For some samples I have the generation of the outuput.bam file while for others no.

gatk --java-options "-Xmx750G" PathSeqPipelineSpark
--input output.bam
--filter-bwa-image ${imagehuman}
--kmer-file ${pathseqdb}/host.hss
--min-clipped-read-length 60
--microbe-dict ${bactdict}
--microbe-bwa-image ${pathseqdb}/bacteria_only.fa.img
--taxonomy-file $taxdb
--output ${outpath}pathseq.complete0.8.bam
--scores-output ${outpath}/pathseq.complete0.8.csv
--is-host-aligned false
--filter-duplicates false
--min-score-identity .8

The path to the related file are correct so I was wondering if someone can help me.

This is the error A USER ERROR has occurred: Couldn't write file pathseq.complete0.8.bam because writing failed with exception No FileSystem for scheme "null"

Best, Martina

martitroiani avatar Jul 11 '24 16:07 martitroiani