Added sequences to a total of 0 reads.

[Arkadiy-Garber]

Getting the following output. Some help would be appreciated.

(deepsignalenv) MAB@Axceleron-WKS:~/2748_NP_methylation/fast5_pass$ tombo preprocess annotate_raw_with_fastqs --fast5-basedir single_barcode12/ --fastq-filenames barcode12/barcode12.guppy.fastq

[16:00:39] Preparing reads and extracting read identifiers. 100%|██████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████| 110340/110340 [03:07<00:00, 587.69it/s] [16:03:48] Annotating FAST5s with sequence from FASTQs. ****** WARNING ****** Some FASTQ records contain read identifiers not found in any FAST5 files or sequencing summary files. 0it [00:01, ?it/s] [16:03:50] Added sequences to a total of 0 reads.

(deepsignalenv) MAB@Axceleron-WKS:~/2748_NP_methylation/fast5_pass$

Thanks, Arkadiy

[Arkadiy-Garber]

For some context, this was the preceding guppy run, which finished without issue:

guppy_basecaller -i barcode12/ -r -s barcode12/ --flowcell FLO-MIN114 --kit SQK-NBD114-24 --cpu_threads_per_caller 16 --resume --as_cpu_threads_per_scaler 16 --num_barcoding_threads 16 --bam_out