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msgf+ needs the disassociation method/fragmentation methods

Open ypriverol opened this issue 8 months ago • 1 comments

Description of feature

SAGE and Comet use the MS level to target identifications, while MSGF+ uses fragmentation mode HCD, CID, etc. In the current version of the pipeline, if we put HCD in the SDRF and the file is CID, we will not identify any peptide. This is now making me go inside the MS/MS file and try to see which method is used at MS2. I think would be good to contact MSGF team and define the spectrum level that would be used for identification, similar to Comet and SAGE, rather than using the fragmentation mode.

Opinions @jpfeuffer @daichengxin @timosachsenberg

ypriverol avatar May 09 '25 09:05 ypriverol

Yes I guess it makes sense to add a level parameter there. Maybe even allow combination with the dissociation type for full flexibility.

jpfeuffer avatar May 09 '25 11:05 jpfeuffer