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Use custom score matrix error?

Open liufuyan2016 opened this issue 1 month ago • 1 comments

Dear, I use custom score matrix below : image and running the below command use diamond v2.1.9.163 : diamond blastp --more-sensitive -d dataset.fa -q query.part_001.fa -p 5 -f 6 qseqid sseqid pident length mismatch ppos gapopen qstart qend sstart send evalue bitscore qlen slen stitle -o dataset.fa.1.blast.out --max-target-seqs 5 --custom-matrix matrix.txt --gapopen 11 --gapextend 1 There are one error:

#CPU threads: 5 Error: The ALP library failed to compute the statistical parameters for this matrix. It may help to adjust the gap penalty settings.

How to solve it? How to set the --gapopen and --gapextend parameter? Thank you very much! I am looking forwards your reply!

liufuyan2016 avatar May 11 '24 09:05 liufuyan2016