Parameter to obtain the most similar sequence

[duongvtt96]

Hi, I would like to obtain the most similar sequence for each query. Is there any parameter in Diamond to do that, instead of setting e-value or bit score? Thank you !

[bbuchfink]

You can use -k1 to report the best hit (in terms of evalue). Sorting by other metrics is not supported, but you can use -k0 to report all hits and then sort them by the metric of your choice.