Diamond behavior when a DB is not explicitly made.

[EnzoAndree]

I have a question.

What happens if I don't explicitly make a DB in diamond and run the search directly against the protein multifasta?

Is a DB generated in RAM anyway? Is there any performance loss?

I hope you can solve this doubt. Best regards Enzo

[bbuchfink]

The database is created in the temp directory as if makedb were called, so it's not recommended to do this for large fasta files.