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Issue with RNA-seq data processing(Step-2)

Open sim3023 opened this issue 11 months ago • 0 comments

I am facing an error during the 2nd step of the pipeline. I suppose the error is related to prefetch and fasterq-dump while fetching data from SRA. Also, I would like to mention that I am using version 22.10.8 of nextflow for running the pipeline as I face errors with the latest version of the same. It would be great if someone could help with the following error.

sudo ./nextflow run main.nf -profile local --organism mycobacterium_abscessus --metadata mab.tsv --sequence_dir sequence_dir/ --outdir results N E X T F L O W ~ version 22.10.8 Launching main.nf [reverent_bartik] DSL1 - revision: ef90b5fca3 executor > local (13) [27/ad4b19] process > bowtie_build [100%] 1 of 1 ✔ [8d/7bec19] process > gff2bed [100%] 1 of 1 ✔ [56/467f86] process > download_fastq (14) [ 1%] 5 of 306, failed: 5, retries: 5 [- ] process > stage_fastq_single - executor > local (13) [27/ad4b19] process > bowtie_build [100%] 1 of 1 ✔ [8d/7bec19] process > gff2bed [100%] 1 of 1 ✔ [56/467f86] process > download_fastq (14) [ 1%] 5 of 306, failed: 5, retries: 5 [- ] process > stage_fastq_single - executor > local (14) [27/ad4b19] process > bowtie_build [100%] 1 of 1 ✔ [8d/7bec19] process > gff2bed [100%] 1 of 1 ✔ [41/5bd9cd] process > download_fastq (20) [ 1%] 6 of 307, failed: 6, retries: 6 [- ] process > stage_fastq_single -

sim3023 avatar Feb 29 '24 12:02 sim3023