spateo-release
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Can I use non cellsplited data to use spateo to make 3D?
Dear, Thanks for your great working for spateo. I want to ask a question. Can I use the level data(bin15) not use cellsplited to make 3D visualization? At present, my adata only have the following: AnnData object with n_obs × n_vars = 4414 × 34688 obs: 'in_tissue', 'array_row', 'array_col', 'slices' var: 'gene_ids', 'feature_types' uns: '__type', 'spatial' obsm: 'spatial' And I want to use the adata.obsm['spatial'] to make Image alignment in FIJI and achieve the xml data, but when we use the adata and alighment image to combine,it showd the slices are not alignmented. so we don't know where wrong. Is there any problem in my working? I would be very grateful to hear from you !