Antônio Camargo
Antônio Camargo
The estimated counts values seem to be correct. What should I send you? A image of my session or just the sleuth object?
Sure, @fataltes! Here's Puffaligner's (using `--bestStrata`) `samtools flagstat` output: ``` 214688504 + 0 in total (QC-passed reads + QC-failed reads) 50488220 + 0 secondary 0 + 0 supplementary 0 +...
Thanks Rob! I just started testing puffalign and I'm really excited with it. I may open some other issue tickets soon :)
Thank you for the answers! I'm not using a grid engine. This execution was performed in an AWS instance. I'll try using less threads in my next assemblies. In any...
@milot-mirdita I reduced the number of cores so that I had ~5GB of memory per thread, but the problem persisted. I got the same error across multiple tries. [plass_assembly.log](https://github.com/soedinglab/plass/files/3682084/plass_assembly.log)
Something else that I've noticed: After finishing the assembly of a sample I started assembling the second one. The "generate k-mers list" step was very slow and eventually died. After...
Sure @martin-steinegger! I'll email the data to you.
Thank you, @martin-steinegger I have 2×150bp reads that do not overlap, so I'm afraid that I won't be able to call genes in such short sequences. Thank you for the...
Hi @mooreryan I classified my sequences into three groups: - Complete (with start and stop codons) - Semi-partial (with a start or a stop codon) - Partial (without start and...
Thanks @martin-steinegger! After our conversation. Got some ideas to improve the NN for small peptides. If you plan to work on that in the future, let me know!