results_transcripts error

[SAMANDOLL]

results_transcripts = stattest(bg_filt, feature="transcript" , covariate = "group" , getFC = TRUE, meas = "FPKM") Coefficients not estimable: libadjust Error in coef[r2, ] <- NA : incorrect number of subscripts on matrix

i am facing this error. kindly guide me

[chichizhao]

hey, friend same question that I meet, after look at the help . I solve this problem with such command line.

results_transcripts = stattest(bg, feature = "transcript", covariate = "type",getFC = TRUE, meas = "FPKM",libadjust = FALSE)

her also my problem

results_transcripts = stattest(bg, feature = "transcript", covariate = "type",getFC = TRUE, meas = "FPKM") Coefficients not estimable: libadjust Error in solve.default(t(mod) %*% mod) : Lapack routine dgesv: system is exactly singular: U[3,3] = 0 In addition: Warning message: Partial NA coefficients for 4564 probe(s) results_transcripts = stattest(bg, feature = "transcript", covariate = "type",getFC = TRUE, meas = "FPKM",libadjust = TRUE) Error in stattest(bg, feature = "transcript", covariate = "type", getFC = TRUE, : is.numeric(libadjust) | identical(libadjust, FALSE) is not TRUE results_transcripts = stattest(bg, feature = "transcript", covariate = "type",getFC = TRUE, meas = "FPKM",libadjust = FALSE)

[chichizhao]

finially i found that the source of this problem may relative to your origion data, here i just use the data that procecced from the same raw data, it is a bit of awkward, but it is that.