Masurca issue mega-reads pass 1 failed

[marco91sol]

Dear all,

I am using Masurca for hybrid genome assembly through its conda version.

I ran masurca config.txt and I received the following error (image attached)

How I could solve this issue?

Thanks for your support!

[alekseyzimin]

Hello, please check your Illumina data, there is an error in the fastq header. The mega-reads failed most likely because of not enough free memory, how much free RAM do you have?

[marco91sol]

These are the values at the moment:

MiB Mem : 515615.4 total, 239152.4 free, 109026.5 used, 167436.5 buff/cache MiB Swap: 0.0 total, 0.0 free, 0.0 used. 403300.1 avail Mem

[alekseyzimin]

Should be enough fo 1.8gbp genome. You can re-generate assemble.sh and re-run. Make sure you have disk space available as well. The code that failed is solid, there have been no reports of failures.

On Fri, Dec 18, 2020 at 9:30 AM marco91sol [email protected] wrote:

These are the values at the moment:

MiB Mem : 515615.4 total, 239152.4 free, 109026.5 used, 167436.5 buff/cache MiB Swap: 0.0 total, 0.0 free, 0.0 used. 403300.1 avail Mem

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