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softclipped parts of reads as considered as indels

Open pavanvidem opened this issue 10 years ago • 0 comments

eXpress seems to treat soft clipping in read as indels. This can be proven with the option --max-indel-size. I have reads with soft clips ranging from 3 to 40 bases. With default --max-indel-size (i.e., 10), express could only quantify reads with max indels <= 10 includin soft clips. I lost most of my perfectly matching reads in quantification because they have soft clipped parts which are longer than 10 bases long . With changing the --max-indel-size to 40 could quantify most of the reads, but sometimes lead to segfault.

pavanvidem avatar Feb 25 '16 17:02 pavanvidem