spades-core: /spades/assembler/src/common/assembly_graph/core/debruijn_data.hpp:38: void debruijn_graph::CoverageData::inc_coverage(int): Assertion `value >= 0 || coverage_ > unsigned(-value)' failed.

Open Amandkaur opened this issue 1 year ago • 1 comments

Description of bug

Greetings! Thank you for the amazing tool. I am having trouble in assembling shotgun metagenomic raw reads of some of the samples. I am not sure what underlying issue is and how to resolve. I have tried different memory allocations and k-mer sizes but every time it terminates at the same step.

spades.log

params.txt

SPAdes version

3.13.0

Operating System

Linux-4.4.0-210-generic-x86_64-with-debian-stretch-sid

Python Version

3.7.9

Method of SPAdes installation

manual

No errors reported in spades.log

[X] Yes

Aug 10 '24 18:08 Amandkaur

You are using ancient version of SPAdes. Does the issue reproduce on the latest SPAdes 4.0? Likely your reads contain over-represented k-mer (e.g. an untrimmed adapter or something like this).

Aug 11 '24 00:08 asl