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Published 20 hours ago • verified publisher icon a-slide

About passed read number

Open wuy24 opened this issue 1 year ago • 0 comments

Describe the bug Hi, I have a direct RNA ONT run. I ran pycoQC using the sequencing_summary file generated by Guppy. From the html file, I can see the passed read number is 170979, however, the actual passed reads in the fastq file is 128456. I do not know why. And I wonder how pycoQC count the passed read number. Attached is the sequencing_symmary file. Coud you please help to take a look? Thank you very much!

Best, Ying sequencing_summary_FAT59544_5f60aeab.txt.zip

wuy24 avatar Oct 07 '22 19:10 wuy24