Cassiopeia
Cassiopeia copied to clipboard
Segmentation fault in cas.pp.align_sequences
Hello! I encountered the following error
joblib.externals.loky.process_executor.TerminatedWorkerError: A worker process managed by the executor was unexpectedly terminated. This could be caused by a segmentation fault while calling the function or by an excessive memory usage causing the Operating System to kill the worker.
in cas.pp.align_sequences when setting the gap_extend_penalty as a float number less then 1. I noticed that in our experiment, the Cas9 will cut a long sequence(about 200-300bp). Therefore, I want the gap extend penalty to be smaller so that it will record the alleles on two distant cutting sites that would otherwise be omitted by local alignment.