Sierra dataframe has 0 length

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Hi everyone:

I met a problem when I run the DUTest as below, so I find my peaks.seurat@tools$Sierra doesn't have any thing except the rownames.

res.table = DUTest(peaks.seurat,

•                population.1 = "W-P", 
  

•                population.2 = "L-P",
  

•                exp.thresh = 0.1, 
  

•                #feature.type = c("UTR3", "exon")
  

•                )
  

[1] "0 expressed peaks in feature types UTR3, exon" [1] "0 genes detected with multiple peak sites expressed" [1] "0 individual peak sites to test" Error in [.data.frame(Tool(apa.seurat.object, "Sierra"), , c("Gene_name", : 选择了未定义的列 And my annotation_peak.txt as below:

I have no idea why it lost the information of Sierra dataframe when combine the seruat object and annotated peaks. Can anyone help me?