SikangSHU

Thanks so much for your patient answers! I have learnt a lot from the code you give and it runs successfully on my computer. While I think the method is...

> > > > I consider to do stain unmixing for the wsi. The picture as follows is a part of a wsi. Thank you!  This is the result...

Dear professor: I think you may do stain unmixing on one channel for three times while there are three channels. (Or maybe my guess is wrong

> I agree, it's not working well and I'm not sure why. I might take another look later today if I have time. Dear Professor: I think the inverse process...

Hello! I need to add a fluorescent color to the image, so I'd like to convert the three channels rgb image(tiff file) to one channel. More specifically, I need to...

> These are OME-TIFFs, right? I would make two one-band images and attach some XML metadata saying that the first is a stain and the second is a florescence image....

`(learn) C:\Users\91765>vipsheader -f image-description E:\\testpicture_jupyter\\channel\\1.tif (vipsheader:3340): VIPS-WARNING **: 22:20:58.807: Unknown field with tag 33560 (0x8318) encountered (vipsheader:3340): VIPS-WARNING **: 22:20:58.817: Unknown field with tag 33560 (0x8318) encountered (vipsheader:3340): VIPS-WARNING **:...

OK. Thank you for your patient answering. This is the information. 

> You'll need to design some XML based on that, then set that as the image description of the TIFF you write. Eg.: > > ```python > final_image = ......

Hi, I’d like to get a pyramidal tiff with which the colourspace is ‘grey16’(As shown in the figure, ). When I write like that in my code, the output image...