cellcall icon indicating copy to clipboard operation
cellcall copied to clipboard

Published 20 hours ago • verified publisher icon ShellyCoder

Normalised data

Open Imm123 opened this issue 1 year ago • 1 comments

Hi, I would like to ask you about normalisation If I have rawCounts, what do you think it is the best way to do it?

Thanks

Imm123 avatar May 09 '23 16:05 Imm123

There are two normalization factors, one is the library size of each cell, the other is gene length. Depend on your sequence technology to correct your data. If your data is UMI scRNAseq which is not affected by gene length, you can jues correct library size with CPM.

It's my opinion, wish it provide some help. Best wishes.

ShellyCoder avatar May 11 '23 01:05 ShellyCoder