Running error: Too many open files

[summerXiaHan]

Hello SchulzLab, I wanna use ORNA to normalize my datasets and I tried it on an fastq file but failed. This fastq file is the RNAseq sample with 150bp read length and ~24 million reads. My command is: ORNA -kmer 25 -input ../00.fastq/PA1_female_1.clean.fq -nb-cores 30 -type fastq -output Normalized

Then the the following errors came out:

How can I resolve this problem? Thank you!

[SchulzLab]

Dear Xia Han, this is a problem with DSK. I found an issue in their GitHub were this was fixed: https://github.com/rundsk/dsk/issues/105

We will look into how to update this as part in ORNA. Kind regards, Marcel