CRISPRCasTyper
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castyping.py having an issue with positional arguments.
I've had cctyper working before on another machine. Not sure if you have seen this error before.
(cctyper)user assembly % cctype CIA1\ copy.fna CIA1_cctyper
zsh: command not found: cctype
(cctyper)user assembly % cctyper CIA1\ copy.fna CIA1_cctyper
/Users/user/miniconda3/envs/cctyper/lib/python3.9/site-packages/Bio/pairwise2.py:278: BiopythonDeprecationWarning: Bio.pairwise2 has been deprecated, and we intend to remove it in a future release of Biopython. As an alternative, please consider using Bio.Align.PairwiseAligner as a replacement, and contact the Biopython developers if you still need the Bio.pairwise2 module.
warnings.warn(
[2023-05-18 10:59:17] INFO: Running CRISPRCasTyper version 1.3.0
[2023-05-18 10:59:18] INFO: Predicting ORFs with prodigal
[2023-05-18 10:59:28] INFO: Running HMMER against Cas profiles
100%|███████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████| 691/691 [01:11<00:00, 9.65it/s]
[2023-05-18 11:01:14] INFO: Parsing HMMER output
[2023-05-18 11:01:14] INFO: Subtyping putative operons
Traceback (most recent call last):
File "/Users/user/miniconda3/envs/cctyper/bin/cctyper", line 80, in
Fixed it by changing single_effector_hmms = self.scores[self.scores['Hmm'].isin(list(specifics))].drop('Hmm', 1) to single_effector_hmms = self.scores[self.scores['Hmm'].isin(list(specifics))].drop('Hmm', axis=1)
Thank you for reporting this. Looks like I have to be more restrictive on the pandas and biopython versions