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BLAST version in fragmentation tools for ab initio

Open NooraAz opened this issue 7 months ago • 1 comments

There is a version incompatibility between the nr library and the BLAST version that causes the following error when running make_fragments.pl:

[fastacmd] ERROR: ERROR: Cannot initialize readdb for nr database

That's because the default nr library is upgraded to V5, but fastacmd can only read V4. There are two options here:

  1. Downgrading nr library
  2. Upgrading BLAST

The new BLAST is faster, but it needs more work and rewriting of code. Downgrading nr is an easier fix.

I want to know your opinion, before working on it, should we downgrade nr or upgrade BLAST? And of course, I would appreciate any help in fixing this.

NooraAz avatar Jul 13 '24 19:07 NooraAz