rosetta
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BLAST version in fragmentation tools for ab initio
There is a version incompatibility between the nr library and the BLAST version that causes the following error when running make_fragments.pl
:
[fastacmd] ERROR: ERROR: Cannot initialize readdb for nr database
That's because the default nr library is upgraded to V5, but fastacmd can only read V4. There are two options here:
- Downgrading nr library
- Upgrading BLAST
The new BLAST is faster, but it needs more work and rewriting of code. Downgrading nr is an easier fix.
I want to know your opinion, before working on it, should we downgrade nr or upgrade BLAST? And of course, I would appreciate any help in fixing this.