Extract RNA and run qPCR of remaining Lifestage Carryover project samples

[AHuffmyer]

We should next extract RNA and run qPCR for the remaining LCO project samples given that we found differential expression in the seed and spat samples run previously. In this last round, we analyzed gene expression in conditioned vs unconditioned seed and spat (Oct exposure) under acute stress conditions (Feb exposure).

There are 80 total samples left. A sheet with the sample information and tube numbers are in a .csv on GitHub here.

These samples would allow us to analyze expression from conditioned vs unconditioned animals (Oct exposure) from adult, spat, seed, and juvenile stages under control or acute stress conditions (Feb exposure).

There is no strict deadline on this - process as we can.

@sr320 would you like these samples to perhaps be saved for a summer student project?

@kubu4 given that we have 80 total samples what is a reasonable timeline to get started? I'd love to learn your extraction and qPCR protocol and help process.