Cases with no polyA

[Kensei-K]

Dear Developers of DeePlexiCon,

I just came across this github repo and the paper. I'd love to try using this approach to multiplex with your barcodes. However, I plan to sequence RNA without polyA tail using custom sequence-specific adapters.

I was wondering if you have an idea on if your algorithm works to demultiplex barcodes in RNA without polyA tail? For example, if your algorithm tries to find signals corresponding to barcodes by finding signals for polyA tail, then I imagine it might not work for my samples without polyA tail?

Thanks in advance!

[noncodo]

It should work ok. There may be some slight issue with classification probabilities resulting from a non poly(rA) sequence after the barcode, but I think this will be negligible given the strong difference in current amplitude between the DNA barcode and the RNA sequence