ROGUE
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Could I use rogue for seurat_clusters and orig.ident ?
Hello,
I have a Smart-seq2 scRNA-seq dataset with multiple samples.
However, I just hope to check whether the resolution parameter is suitable or not for the whole dataset rather than each sample. So I use orig.ident (all are same) in "samples" parameter.
I use rogue as follow:
rogue(seurat@assays$RNA@data, labels = seurat$seurat_clusters, samples = seurat$orig.ident, platform = "full-length", span = 0.5)
Is it correct ?